On the planet of superior fat-loss methods, two compounds have been producing growing curiosity: 5-Amino-1MQ and AOD-9604. Whereas each are designed to assist a leaner physique, they work by way of very completely different mechanisms — one by way of metabolic enzyme inhibition and the opposite by way of a progress hormone–derived fat-burning fragment.
Why this comparability issues: Understanding how every compound works and the place it excels may help athletes, bodybuilders, and physique-focused people resolve whether or not to make use of one, the opposite, or each collectively for complementary fat-loss pathways.
On this information, we’ll break down:
→ What every compound is and the way it works.
→ Key advantages for fats loss, efficiency, and muscle preservation.
→ Stacking and biking methods.
→ Unwanted side effects, security, and authorized concerns.
By the tip, you’ll have a transparent, evidence-backed framework for deciding which compound finest matches your objectives, coaching section, and aggressive standing.
What Is 5-Amino-1MQ?
5-Amino-1-methylquinolinium (5-Amino-1MQ) is a small-molecule inhibitor of the enzyme nicotinamide N-methyltransferase (NNMT)—a metabolic regulator tied to fats storage, NAD⁺ depletion, and decreased vitality expenditure. By blocking NNMT, 5-Amino-1MQ helps protect nicotinamide adenine dinucleotide (NAD⁺), a coenzyme important for mitochondrial vitality manufacturing, metabolic flexibility, and fats oxidation.
Key physiological results embrace:
→ Elevated basal metabolic charge (BMR)
→ Enhanced mitochondrial effectivity and endurance
→ Fats loss whereas preserving lean muscle mass
For a full breakdown of NNMT inhibition, NAD⁺ preservation, and the function of SIRT1, see 5-Amino-1MQ for Newbies: Dosage, Advantages, and Peptide Stacks Defined.
Whereas gaining traction in efficiency circles, 5-Amino-1MQ stays an experimental analysis compound and is not FDA-approved for human use.
What Is AOD-9604?
AOD-9604 is an artificial peptide fragment derived from the C-terminal (176–191) sequence of human progress hormone (HGH). Not like full-length HGH, AOD-9604 is non-anabolic — it doesn’t enhance IGF-1 ranges or stimulate muscle progress — nevertheless it retains and amplifies the fat-burning (lipolytic) properties of HGH.
Key physiological results embrace:
→ Stimulates lipolysis — the breakdown of saved triglycerides into free fatty acids.
→ Inhibits lipogenesis — the conversion of dietary carbohydrates into saved physique fats.
→ Targets cussed fats deposits (stomach and visceral fats specifically).
Analysis means that AOD-9604 promotes fats loss with out the fluid retention or carbohydrate metabolism modifications typically related to HGH remedy.
As a result of it acts particularly on adipose tissue, AOD-9604 is usually utilized in slicing protocols, physique recomposition plans, and as a longer-term metabolic assist peptide as a result of its favorable security profile.
Mechanism of Motion Comparability
Though 5-Amino-1MQ and AOD-9604 each assist fats loss, their organic pathways are totally completely different.
→ 5-Amino-1MQ
5-Amino-1MQ works by inhibiting nicotinamide N-methyltransferase (NNMT), an enzyme that regulates NAD⁺ metabolism and influences fats storage. By blocking NNMT, the physique is ready to:
→ Protect and elevate NAD⁺ ranges, which reinforces mitochondrial vitality manufacturing.
→ Activate SIRT1, a protein that promotes fats oxidation, glucose regulation, and muscle preservation.
→ Improve basal metabolic charge (BMR), resulting in greater calorie burn at relaxation.
For an in depth breakdown of this pathway, see 5-Amino-1MQ for Newbies: Dosage, Advantages, and Peptide Stacks Defined.
→ AOD-9604
AOD-9604 is a 176–191 fragment of human progress hormone that particularly targets fats metabolism with out influencing anabolic processes. It really works by:
→ Stimulating lipolysis — breaking down saved triglycerides into free fatty acids for vitality.
→ Inhibiting lipogenesis — stopping the storage of dietary fat.
→ Appearing instantly on adipose tissue with out affecting insulin sensitivity or IGF-1 manufacturing.
Research present AOD-9604 can cut back cussed fats deposits, significantly visceral and stomach fats, whereas sustaining a positive security profile in longer-term use.
Advantages Comparability
Though 5-Amino-1MQ and AOD-9604 are each used to advertise fats loss, they act by way of completely different main mechanisms — giving them distinctive strengths that can be utilized alone or in synergy.
→ 5-Amino-1MQ
Finest For: Maximizing metabolic charge, enhancing endurance, and selling fats oxidation whereas preserving lean muscle mass.
1. Elevated Basal Metabolic Price (BMR)
By inhibiting NNMT, 5-Amino-1MQ preserves NAD⁺, which helps mitochondrial effectivity and thermogenesis — growing resting calorie burn even throughout caloric restriction.
As famous by Kraus et al., Nature Medication, NNMT inhibition in white adipose tissue considerably elevated vitality expenditure and decreased fats mass in animal fashions with out modifications to weight loss plan or exercise.
2. Muscle Preservation Throughout Caloric Deficit
Larger NAD⁺ availability prompts sirtuin pathways, enhancing protein retention and lowering muscle breakdown.
Worth et al., Cell Metabolism report that SIRT1 activation improves mitochondrial operate, reduces irritation, and protects lean mass beneath metabolic stress.
3. Enhanced Metabolic Flexibility
The NAD⁺–SIRT1–PGC-1α axis improves the physique’s potential to effectively change between carbohydrate and fats gasoline sources.
In keeping with Cantó et al., Cell Metabolism, elevated NAD⁺ stimulates PGC-1α exercise, driving mitochondrial biogenesis and substrate flexibility.
4. Endurance & Coaching Output
Extra environment friendly mitochondria imply better ATP output for prolonged efficiency.
Kannt & Pfenninger, Frontiers in Physiology emphasize that NAD⁺ preservation enhances oxidative metabolism, supporting sustained train capability.
→ AOD-9604
Finest For: Focused fats discount, long-term fats administration, and cussed fats mobilization with out anabolic or stimulant negative effects.
1. Direct Motion on Adipose Tissue
AOD-9604 mimics the fat-burning area of HGH (176–191) and instantly stimulates fats breakdown whereas blocking fats storage.
Ng et al., Journal of Endocrinology discovered that AOD-9604 stimulates lipolysis and inhibits lipogenesis in adipose tissue with out impacting growth-promoting pathways.
2. Discount of Cussed Fats Shops
Notably efficient for stomach and visceral fats — essentially the most proof against weight loss plan and coaching alone.
Ng et al., Journal of Endocrinology noticed focused reductions in fats deposits with out opposed metabolic results.
3. Minimal Impression on Hormonal Stability
Avoids the systemic hormonal results and water retention typically seen with full HGH use.
As reported by Ng et al., Journal of Endocrinology, AOD-9604 doesn’t enhance IGF-1 ranges, making it safer for long-term use.
4. Prolonged Use Potential
Resulting from its non-anabolic nature, AOD-9604 can typically be run longer than conventional fat-loss compounds.
5. Non-Stimulant Fats Loss
Promotes fats discount with out CNS stimulation, making it appropriate for night use and for people delicate to stimulants.
Stacking Potential
As a result of 5-Amino-1MQ and AOD-9604 act by way of completely different fat-loss pathways, they are often mixed for synergistic outcomes — one boosting general metabolic charge and mitochondrial effectivity, the opposite instantly focusing on fats cell metabolism.
→ 5-Amino-1MQ + AOD-9604 for Fats Loss Synergy
Stacking these two compounds can:
→ Speed up fats oxidation (by way of NAD⁺ preservation and SIRT1 activation).
→ Improve calorie burn at relaxation (BMR elevation).
→ Mobilize cussed fats deposits (direct lipolysis and anti-lipogenesis).
As famous by Kraus et al., Nature Medication and Ng et al., Journal of Endocrinology, focusing on each systemic metabolism and localized fats cell exercise could improve fat-loss outcomes past both method alone.
→ Including Endurance & Conditioning Help
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Cardarine (GW-501516) – Improves fatty acid utilization throughout train and enhances cardio capability, complementing mitochondrial advantages from 5-Amino-1MQ.
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CJC-1295 vs Ipamorelin – Stimulates pure progress hormone launch, aiding restoration and fats loss.
→ Enhancing Restoration & Tissue Well being Throughout a Reduce
→ Dietary & Hormonal Help
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DHEA – Helps hormone steadiness and vitality throughout calorie restriction.
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Krill Oil – Gives omega-3 fatty acids for irritation management and metabolic well being.
Biking Issues
Though 5-Amino-1MQ and AOD-9604 might be stacked, their optimum cycle lengths differ based mostly on their mechanisms and tolerability profiles. Understanding these variations helps keep away from metabolic adaptation and ensures constant outcomes.
→ 5-Amino-1MQ Biking
Most protocols run 4–6 weeks on adopted by 2–4 weeks off. This off interval permits the physique’s enzymatic methods — particularly NNMT exercise — to reset, sustaining responsiveness.
Kraus et al., Nature Medication spotlight that sustained NNMT inhibition drives metabolic modifications, however strategic breaks could assist protect efficacy over time.
→ AOD-9604 Biking
AOD-9604 has a positive security profile and is typically used for longer durations — generally 12+ weeks — as a result of its non-anabolic, focused mechanism. Nevertheless, periodic breaks can nonetheless assist assess progress and guarantee continued effectiveness.
In keeping with Ng et al., Journal of Endocrinology, AOD-9604 acts instantly on adipose tissue metabolism with out influencing IGF-1 or systemic hormone ranges, supporting longer-term protocols.
→ Integrating the Two
When stacking:
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Begin each compounds concurrently throughout a slicing section for max synergy.
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Run 5-Amino-1MQ in outlined cycles inside an extended AOD-9604 program.
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Monitor physique composition and coaching efficiency to regulate timing or introduce restoration phases.
Aligning cycles with coaching blocks — for instance, beginning each originally of a calorie deficit or contest prep — can amplify outcomes whereas preserving muscle and vitality.
Authorized Standing
Each 5-Amino-1MQ and AOD-9604 are not authorised by the U.S. Meals and Drug Administration (FDA) for human use. They’re usually marketed as analysis compounds and bought beneath “for analysis functions solely” disclaimers.
→ 5-Amino-1MQ
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Regulatory Standing: Categorised as an experimental small molecule; can’t be legally marketed as a dietary complement or treatment.
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Sport Compliance: Falls beneath the World Anti-Doping Company’s (WADA) S0 classification, that means all non-approved substances are prohibited in competitors.
WADA specifies in its S0 clause that any pharmacological substance with out regulatory approval for human use is banned always in sport (World Anti-Doping Company).
→ AOD-9604
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Regulatory Standing: Additionally not FDA-approved; permitted on the market solely as a analysis peptide.
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Sport Compliance: Like 5-Amino-1MQ, AOD-9604 is prohibited beneath WADA’s S0 class.
In keeping with the Australian Sports activities Anti-Doping Authority, AOD-9604 is particularly listed as prohibited for athletes beneath the S0 “Non-Accredited Substances” rule (ASADA).
→ Key Implications
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Aggressive Athletes: Each compounds must be averted if topic to drug testing, as a constructive end result can happen even with out specific naming on the prohibited checklist.
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Jurisdictional Variation: Some nations could classify these as prescription-only medicines, whereas others deal with them solely as unregulated analysis chemical compounds.
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Sourcing Dangers: Buying from unverified suppliers will increase the chance of contamination, mislabeling, or unlawful distribution.
Which Ought to You Select?
Deciding between 5-Amino-1MQ and AOD-9604 will depend on your objectives, coaching section, and tolerance for analysis compounds. Whereas each goal fats loss, their mechanisms and utility methods differ considerably.
→ Select 5-Amino-1MQ If:
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You need to enhance general metabolic charge and calorie burn at relaxation.
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Preserving lean muscle mass throughout a calorie deficit is a precedence.
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You’re centered on endurance efficiency or metabolic flexibility.
Kraus et al., Nature Medication present that NNMT inhibition can enhance vitality expenditure and enhance metabolic well being with out altering weight loss plan or exercise.
→ Select AOD-9604 If:
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Your essential aim is focusing on cussed fats deposits like stomach or visceral fats.
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You like a longer-term, non-stimulant fat-loss method.
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You need fats loss with out altering systemic hormone ranges.
Ng et al., Journal of Endocrinology discovered that AOD-9604 stimulates lipolysis and inhibits fats storage with out growing IGF-1 or inflicting anabolic results.
→ Stack Each If:
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You need to assault fats loss from a number of angles — systemic metabolism and localized fats breakdown.
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You’re in a quick, aggressive slicing section and wish most synergy.
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You may decide to correct biking: 5-Amino-1MQ in outlined blocks inside an extended AOD-9604 program.
Combining compounds that act by way of completely different pathways — NAD⁺ preservation and mitochondrial effectivity for 5-Amino-1MQ, direct fats cell modulation for AOD-9604 — can create a extra sturdy fat-loss atmosphere than utilizing both alone.
| Issue | 5-Amino-1MQ | AOD-9604 |
|---|---|---|
| Main Advantages | → Boosts basal metabolic charge → Preserves lean muscle throughout calorie deficits → Enhances endurance and metabolic flexibility (Kraus et al., Nature Medication) | → Targets cussed fats deposits → Stimulates lipolysis & inhibits fats storage → Non-stimulant, no anabolic hormone results (Ng et al., Journal of Endocrinology) |
| Typical Dose | 50–100 mg per day, divided into 1–2 doses Cycle: 4–6 weeks on, 2–4 weeks off | 250–500 mcg each day Typically taken for 12–24 weeks for finest outcomes |
| Frequent Aspect Results | Delicate complications, transient nausea, potential modifications in blood stress; long-term security not established | Delicate injection web site irritation, complications, momentary urge for food modifications; typically effectively tolerated in trials |
5-Amino-1MQ vs AOD-9604: Key Takeaways
5-Amino-1MQ and AOD-9604 are each highly effective fat-loss brokers — however their strengths come from totally completely different mechanisms:
→ 5-Amino-1MQ — Inhibits NNMT to protect NAD⁺, activate SIRT1, elevate basal metabolic charge, and enhance metabolic flexibility (Kraus et al., Nature Medication).
→ AOD-9604 — Mimics the fat-burning fragment of HGH (176–191) to stimulate lipolysis, inhibit lipogenesis, and goal cussed fats with out anabolic results (Ng et al., Journal of Endocrinology).
Finest Functions:
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5-Amino-1MQ — Slicing phases, metabolic assist, and muscle preservation.
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AOD-9604 — Cussed fats discount, long-term fats administration, and non-stimulant fats loss.
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Stack — Aggressive short-term fats loss focusing on each systemic and localized fats metabolism.
For athletes and opponents, keep in mind each compounds are prohibited beneath WADA’s S0 class, and neither is FDA-approved for human use.
Disclaimer
This text is for academic functions solely and doesn’t represent medical recommendation. 5-Amino-1MQ and AOD-9604 are investigational analysis compounds and are not authorised by the U.S. Meals and Drug Administration (FDA) for human use.
At all times seek the advice of with a licensed healthcare supplier earlier than starting any supplementation or performance-enhancement protocol. Particular person responses range, and potential interactions with medicines or pre-existing situations have to be evaluated by a professional skilled.
Key Factors:
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Each compounds are labeled as non-approved substances beneath the World Anti-Doping Company’s S0 class and are prohibited in sport.
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Sourcing from unverified suppliers carries dangers of contamination, mislabeling, or inaccurate dosing.
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Neither compound is meant to diagnose, deal with, treatment, or stop any illness.
If you’re searching for research-backed, compliant options for fats loss and efficiency, think about Swolverine’s clinically dosed dietary supplements resembling Krill Oil for irritation management, Creatine Monohydrate for power and restoration, and DHEA for hormonal assist — all third-party examined for purity and label accuracy.